A probable novel splicing isoform of human vascular endothelial growth factor.
- Author:
Zhongjiang ZHOU
1
;
Haiyan YE
;
Kai CUI
;
Xianghui CHEN
;
Yili LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alternative Splicing; Amino Acid Sequence; Exons; Frameshift Mutation; Gene Expression; Humans; Protein Isoforms; classification; genetics; Reverse Transcriptase Polymerase Chain Reaction; Vascular Endothelial Growth Factor A; classification; genetics
- From: Journal of Southern Medical University 2012;32(6):755-759
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo characterize a new alternative splicing isoform of human vascular endothelial growth factor (VEGF) gene.
METHODSThe total RNA was extracted from the lung tissue of a legally aborted 4-month-old fetus and amplified by RT-PCR. The amplified product was cloned into the plasmid pMD18-T and plasmid pcDNA3.1- for sequence analysis.
RESULTSElectrophoresis of the RT-PCR products displayed one short band for VEGF(121) (487 bp) and a long band. The latter was characterized to contain two fragments: one was normal VEGF(165) (619 bp), and the other (639 bp) had an identical nucleotide sequence to VEGF(165) with a 20 bp fragment inserted between exons 3 and 4. Sequence analysis showed that this 20-bp nucleotide was inserted from the 3' end of the third intron containing a splicing signal, thus causing shift mutation in the reading frame of VEGF gene and early appearance of the stop codon UAG in the middle of exon 4.
CONCLUSIONA new alternative splicing isoform of VEGF probably exists in the lung tissue of a legally aborted human fetus, and its biological significance remains to be further investigated.
