Effect of lentiviral vector-mediated short hairpin RNA targeting survivin against endometriosis-like lesions in chick embryo chorioallantoic membrane.
- Author:
Dongxian PENG
1
;
Yuanli HE
;
Liwen QIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chick Embryo; Chorioallantoic Membrane; pathology; Disease Models, Animal; Endometriosis; genetics; Female; Genetic Vectors; Humans; Inhibitor of Apoptosis Proteins; genetics; Lentivirus; genetics; Neovascularization, Pathologic; pathology; RNA Interference; RNA, Small Interfering; genetics
- From: Journal of Southern Medical University 2012;32(7):995-999
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on angiogenesis and growth of endometriosis-like lesions in chick en embryo chorioallantoic membrane.
METHODSEutopic endometrium from women with endometriosis was transplanted onto the non-vascular region of (CAM), where LV-survivin shRNA was delivered subsequently. The angiogenesis and the growth of endometriosis-like lesions in the CAM model were evaluated.
RESULTSThe angiogenesis and formation of endometriosis-like lesions were significantly suppressed in the CAM model by treatment with LV-survivin shRNA in comparison with those in the untreated CAM models and models treated with empty LV or DMEM (P<0.001). LV-survivin shRNA also caused a significantly higher cell apoptotic rate in the endometriosis-like lesions than the other treatments (P<0.001) and induced necrosis in the lesions.
CONCLUSIONLV-survivin shRNA can effectively inhibit angiogenesis induced by the eutopic endometrium and markedly suppress the formation of endometriosis-like lesions in the CAM model.
