Changes of fibronectin and type IV collagen expression in cultured rat mesangial cells transfected with connective tissue growth factor expression vector.
- Author:
Xiao-gang WANG
1
;
Hui-juan WU
;
Qi CHEN
;
Zhong-hua ZHAO
;
Zhi-gang ZHANG
;
Mu-yi GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cells, Cultured; Collagen Type IV; genetics; metabolism; Connective Tissue Growth Factor; genetics; metabolism; Fibronectins; genetics; metabolism; Genetic Vectors; genetics; Mesangial Cells; cytology; metabolism; RNA, Messenger; genetics; metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Chinese Journal of Pathology 2006;35(9):555-558
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of connective tissue growth factor (CTGF) in the development of glomerulosclerosis by experimental alteration of fibronectin (FN) and Type IV collagen (Col IV) expression in cultured rat mesangial cells (MsC).
METHODSCTGF expression vector was transfected into MsC by Lipofectimine method. Protein and mRNA expression levels of CTGF, FN and Col IV were studied by Western blot and reverse transcription-polymerase chain reaction (RT-PCR) respectively.
RESULTSTwo of MsC clones (MCT-1 and MCT-2) with CTGF overexpression were successfully established and found to have significant increases of FN and Col IV at both protein and mRNA levels. Compared with the controls, the expression of FN protein and mRNA in the two clones were 3.2 times (P < 0.05) and 2.9 times (P < 0.05) higher respectively. The expression of Col IV protein and mRNA was 3.8 times (P < 0.01) and 2.4 times (P < 0.01) higher respectively.
CONCLUSIONCTGF up-regulates FN and Col IV expression in MsC and may play an important role in the development of glomerulosclerosis.