Study on the molecular mechanism of endostatin and doxycycline in suppressing melanoma growth.
- Author:
Bao-cun SUN
1
;
Shi-wu ZHANG
;
Li-sha QI
;
Dan-fang ZHANG
;
Hua GUO
;
Xiu-lan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Cell Line, Tumor; Doxycycline; pharmacology; Drug Synergism; Endostatins; pharmacology; Female; Immunohistochemistry; Male; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; Matrix Metalloproteinases; metabolism; Melanoma, Experimental; metabolism; pathology; prevention & control; Mice; Mice, Inbred C57BL; Neoplasm Transplantation; Tissue Inhibitor of Metalloproteinase-2; metabolism; Tumor Burden; drug effects
- From: Chinese Journal of Pathology 2006;35(11):677-680
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular mechanism of endostatin and doxycycline effect on melanoma growth.
METHODSA B16 melanoma mice model was established by intracutaneous injection of B16 cell suspension. The mice were treated with endostatin, doxycycline, endostatin and doxycycline respectively, the control group received no treatment. A time course study of tumor volume was performed to observe the antitumor effect. The expression of matrix metalloproteinase (MMP-9), MMP-2, TIMP-2 were examined by immunohistochemistry staining.
RESULTSTumors in endostatin treatment group, doxycycline treatment group, endostatin and doxycycline treatment group grew slower than in the control group. The difference of the average tumor volume in the doxycycline group and control group, in the doxycycline with endostatin treatment group and control group were statistically different. The positive expression ratio of MMP-2, MMP-9, TIMP-2 in each treatment group were statistically different from their control groups (F = 12.79, F = 5.56, F = 4.64; P < 0.05).
CONCLUSIONDoxycycline and endostatin are able to inhibit the expression of MMPs and promote expression of TIMP, which ultimately inhibits the growth of B16 melonoma.
