Measurement of airway resistance and reactivity in guinea pigs using double-chamber plethysmography.
- Author:
Wei-min YAO
1
;
Ke-fang LAI
;
Yuan-ming LUO
;
Chun-li LIU
;
Ru-chong CHEN
;
Wei LUO
;
Nan-shan ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Airway Resistance; Animals; Asthma; chemically induced; physiopathology; Bronchial Hyperreactivity; etiology; physiopathology; Guinea Pigs; Male; Methacholine Chloride; Plethysmography; instrumentation; methods; Random Allocation
- From: Journal of Southern Medical University 2009;29(5):841-843
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for measurement of airway resistance (sRaw) and reactivity in guinea pigs.
METHODSMethacholine spray at gradient concentrations was given to guinea pigs. PC100 was defined as the concentration of methacholine when the sRaw doubled in the guinea pigs using a double-chamber plethysmograph. The time for the recovery of PC100 resistance to baseline levels was measured. The sRaw and PC100 were measured twice on days 1 and 15 (4 time points) in the guinea pigs before and after OVA challenge.
RESULTSPC100 in a normal guinea pig airway was shown to recover the baseline level within 1 h. Double-chamber plethysmographical measurement of the sRaw and PC100 in normal guinea pigs did not show significant differences between the time points [sRaw: 3.25-/+0.67, 3.33-/+0.58, 3.30-/+0.56, and 3.32-/+0.75 cm H2O.s; log2PC100: 8.48-/+0.94, 8.64-/+1.04, 8.56-/+0.67, and 8.64-/+0.60, respectively, P>0.05]. The sRaw and airway reactivity were significantly increased in guinea pigs challenged with OVA [sRaw: 7.08-/+1.82 vs 2.87-/+0.53 cmH2O.s, P<0.01; log2PC100: 6.64-/+1.26 vs 8.48-/+1.17, P<0.01].
CONCLUSIONA double-chamber plethysmography for measurement of sRaw and airway reactivity in guinea pig is established successfully.
