Effect of sequential intratumoral injection of xenogeneic antigens for immunotherapy in immunized mice bearing S180 tumor.
- Author:
Li-bin SUN
1
;
Ji-ren ZHANG
;
Xi-gang HU
;
Yan-tao MAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Heterophile; immunology; CD4-CD8 Ratio; Female; Immunotherapy; methods; Lymphocytes, Tumor-Infiltrating; cytology; Male; Mice; Random Allocation; Sarcoma 180; immunology; therapy; Streptococcus; immunology
- From: Journal of Southern Medical University 2009;29(5):856-863
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice.
METHODSSequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA.
RESULTSNo significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05).
CONCLUSIONSequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.
