Effect of KDR recombinant adenovirus containing double suicide gene on the proliferation of human colon adneocarcinoma SW620 cells.
- Author:
Yue-dong LIU
1
;
Sen-ming WANG
;
Zong-hai HUANG
;
Qiang LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Adenoviridae; genetics; metabolism; Cell Line, Tumor; Cell Proliferation; drug effects; Colonic Neoplasms; pathology; Cytosine Deaminase; genetics; Genes, Transgenic, Suicide; genetics; Genetic Vectors; genetics; Green Fluorescent Proteins; genetics; metabolism; Humans; Promoter Regions, Genetic; genetics; Recombinant Fusion Proteins; genetics; metabolism; Thymidine Kinase; genetics; Transfection; Vascular Endothelial Growth Factor Receptor-2; genetics; metabolism
- From: Journal of Southern Medical University 2009;29(5):887-893
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of adenovirus (Ad)-mediated fusion gene system driven by the KDR promoter on the proliferation of human colon adenocarcinoma SW620 cells.
METHODSThe KDR-expressing SW620 cells and LS174T cells not expressing KDR were both infected with AdEasy-KDR-CDglyTK followed by treatment with the prodrugs 5-FC and/or ganciclovir at different concentrations. The effect of the transfection on the cell proliferation was evaluated.
RESULTSThe expression of green fluorescent protein (GFP) was observed in 95% of the infected SW620 and LS174T cells with a multiplicity of infection (MOI) of 100. Significant difference was not founded in the growth of SW620 and LS174T cells with or without the transfection. The infected SW620 cells exhibit high sensitivity to the prodrugs, but the infected LS174T cells did not (P<0.01). The CDglyTK fusion gene produced much stronger killing effect of on the target cells than either of the single suicide genes (P<0.01).
CONCLUSIONCDglyTK fusion gene system driven by the KDR promoter selectively kills the KDR-CDglyTK SW620 cells and inhibits the cell proliferation.
