Effect of small interfering RNA targeting CXCR4 on breast cancer angiogenesis.
- Author:
Hong-yuan LI
1
;
Guo-sheng REN
;
Jin-xiang TAN
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; blood supply; genetics; Cell Line, Tumor; Coculture Techniques; Endothelial Cells; cytology; Female; Humans; Neovascularization, Pathologic; genetics; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Receptors, CXCR4; genetics; metabolism; Umbilical Veins; cytology
- From: Journal of Southern Medical University 2009;29(5):954-958
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of a small interfering RNA targeting CXCR-4 (shRNA-CXCR4) on angiogenesis of human breast cancer cells.
METHODSThe expression of CXCR4 mRNA and protein in 3 breast cancer cell lines with CXCR-4 silencing mediated by shRNA-CXCR4 was detected by RT-PCR and Western blotting, respectively. The morphological changes of human umbilical vein endothelial cells (HUVECs) were observed in co-culture with human breast cancer cells after CXCR4 gene silencing.
RESULTSCXCR4 mRNA and protein expressions decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells after the gene silencing (P<0.05). Gene silencing with shRNA-CXCR4 in human breast cancer cells significantly inhibited the ability of HUVECs to form tubular structures in the co-culture (P<0.05).
CONCLUSIONGene silencing by shRNA-CXCR4 can obviously lower the angiogenesis-inducing ability of human breast cancer cells.
