Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO2 extraction.
- Author:
Zhao-yu WANG
1
;
Fei-long CHEN
;
Jing-ming LIN
;
Shu-lin HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Supercritical Fluid; methods; Gas Chromatography-Mass Spectrometry; Jatropha; chemistry; Plant Extracts; analysis; isolation & purification; Plant Leaves; chemistry
- From: Journal of Southern Medical University 2009;29(5):1002-1007
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS).
METHODSThe bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS.
RESULTSSeventy peaks were detected by GC-MS, and 43 compounds were identified (61.43%). Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigmasterol (16.14%), alpha-tocopherol (15.18%), beta-amylin (7.73%) and dotriacontanol (7.02%). The content of gamma-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract.
CONCLUSIONJ. curcas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities.
