Effect of p21-activated kinase-1 on the invasiveness of colorectal carcinoma cells in vitro.
- Author:
Jin-Bao WU
1
;
Yu-Jing HAN
;
Qing-Zhen NAN
;
Zhen-Shu ZHANG
;
Hong-Quan ZHANG
;
Yu-Gang SONG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Colorectal Neoplasms; genetics; pathology; Gene Expression; Genetic Vectors; Humans; Neoplasm Invasiveness; Neoplasm Metastasis; Plasmids; Transfection; p21-Activated Kinases; genetics
- From: Journal of Southern Medical University 2009;29(7):1341-1343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of p21-activated kinase-1 (PAK1) gene transfection on the invasiveness of human colorectal carcinoma SW480 cells in vitro.
METHODSSW480 cells in routine culture were transfected with the recombinant plasmid EGFP-C1/PAK1 via Lipofectamine(TM) 2000. The expression of PAK1 protein in SW480 cells was detected using Western blotting, and the changes of the invasiveness of SW480 cells were evaluated using Boyden chamber invasion assay.
RESULTSForty-eight hours after transfection with pEGFP-C1/ PAK1, the PAK1 protein expression increased significantly in comparison with those in negative and vector control groups. The invasiveness of the SW480 cells was significantly enhanced after the transfection.
CONCLUSIONThe PAK1 gene transfection can increase the expression of PAK1 in SW480 cells and enhance the invasiveness of the cells. PAK1 can be associated with the invasiveness and metastasis of colorectal carcinoma cells.
