Effect of androsterone on scavenger receptor class B type I in human vascular endothelial cells.
- Author:
Fan CHAO
1
;
Wei GONG
;
Yuan LI
;
Zheng-Zhen XU
;
Ming GAO
;
Feng-Tian HE
Author Information
- Publication Type:Journal Article
- MeSH: Androsterone; pharmacology; Cell Line; Endothelial Cells; drug effects; metabolism; Humans; Receptors, Cytoplasmic and Nuclear; agonists; metabolism; Scavenger Receptors, Class B; metabolism
- From: Journal of Southern Medical University 2009;29(7):1344-1347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of FXR on scavenger receptor class B type I (SR-BI) expression.
METHODSHuman vascular endothelium Eahy926 cells were treated with FXR agonist androsterone, and the specific target gene of FXR SHP mRNA was detected by RT-PCR. SR-BI mRNA and protein were determined using RT-PCR, real-time PCR and Western blotting.
RESULTSThe level of SHP mRNA in Eahy926 cells increased after androsterone treatment at different concentrations for 24 h, demonstrating FXR activation in the cells. RT-PCR, real-time PCR and Western blotting detected increased SR-BI expression at both mRNA and protein levels after FXR activation.
CONCLUSIONFXR increases the expression of SR-BI in human vascular endothelium cells.
