Regulation of c-fos gene expression in hepatic stellate cells by transforming growth factor beta.
- Author:
Ai-Min WANG
1
;
Chun-Gai REN
;
Zhi-Jie MA
;
Ying XIANG
;
Zhi-Wei ZHANG
;
Zhi-Jian ZHU
;
Bao-En WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Gene Expression; drug effects; Genes, fos; Hepatic Stellate Cells; drug effects; Proto-Oncogene Proteins c-fos; genetics; metabolism; Rats; Transforming Growth Factor beta; pharmacology
- From: Journal of Southern Medical University 2009;29(7):1408-1409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of transforming growth factor beta (TGF ) on c-fos gene expression in hepatic stellate cells.
METHODSHepatic stellate cells (HSC-T6) were cultured in the medium containing different concentrations of TGF (0.2, 1, and 5 ng/ml), and cells were collected at different time points of incubation (8, 24, 48, and 72 h). The total RNA of the HSCs was isolated and c-fos gene expression level were measured by reverse transcription polymerase chain reaction.
RESULTSc-fos gene expression levels of HSCs cultured in the presence of low (0.2 ng/ml), moderate (1 ng/ml) and high (5 ng/ml) concentrations of TGF for 8, 24, 48 and 72 h were significantly greater than those of control group. The c-fos gene expression levels of HSCs increased gradually with the increment of TGF concentration, and significant differences in c-fos gene expression were found between the 3TGF groups.
CONCLUSIONTGF strongly up-regulates c-fos gene expression in hepatic stellate cells.
