Effect of photodynamic therapy on the proliferation and cell distribution of hypertrophic scar fibroblast.
- Author:
Hong CAI
1
;
Ying GU
;
Qiang SUN
;
Jing ZENG
;
Shi-Long LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Cicatrix; drug therapy; pathology; Fibroblasts; drug effects; metabolism; pathology; Humans; Photochemotherapy
- From: Chinese Journal of Plastic Surgery 2010;26(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of HMME-PDT on the proliferation and cell distribution of hypertrophic scar fibroblast (HSF).
METHODSHSF were cultured and only 4-6th passages were used in this study. Argyrophilic protein in nucleolar organizer regions(AgNORs) were calculated by I. S% after argyrophilic staining. Flow cytometry was applied to analyze the cell cycle and proliferation index (PI).
RESULTS1) I. S% of HSF after HMME-PDT was reduced markedly. 2) HMME-PDT inhibited HSF entering S stage from G, stage, cell percentage in S stage was decreased to (11.2 +/- 2.3)%. 3) PI in HMME-PDT group was less than that in control group [(35.0 +/- 3.4)% vs (27.2 +/- 3.1)%, P < 0.05].
CONCLUSIONSHMME-PDT can inhibit proliferation of HSF, and chang cell distribution.
