The extracellular signal-regulated kinase was promoted by pyrroloquinoline quinine in cultured Schwann cells.
- Author:
Bin HE
1
;
Shi-qing LIU
;
Hao-huan LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Extracellular Signal-Regulated MAP Kinases; metabolism; physiology; Mitogen-Activated Protein Kinases; metabolism; physiology; Pyrroles; pharmacology; Quinolines; pharmacology; Rats; Rats, Sprague-Dawley; Schwann Cells; cytology; drug effects; Signal Transduction
- From: Chinese Journal of Plastic Surgery 2010;26(6):444-447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of mitogen-activated protein kinase (MEK) kinase cascade, extracellular signal-regulated kinase (ERK1/2) signal pathway on Schwann cells proliferation promoted by Pyrroloquinoline Quinine (PQQ) and its molecular mechanisms.
METHODSSchwann cells were cultured and purified in vitro. The purity was identified by S-100. Different time and concentration of PQQ was added into culture medium. The expression of ERK1/2 and phosphorylated-ERK1/2 was detected by western blot. The expression of p-ERK1/2 after blocking of MEK signal pathway by specific inhibitor PD98059 was detected by western blot.
RESULTSMorphological change was observed in PQQ treated Schwann cells. 1-500 nmol/L PQQ could up-regulate the expression of p-ERK1/2, and 1000 nmol/L had no effects, while 10 000 nmol/L exhibited inhibitory effect (P < 0.05). p-ERK1/2 increased to peak 1 h after PQQ added, and this up-regulation of p-ERK1/2 was inhibited by PD98059 (P < 0.05).
CONCLUSIONSPQQ could affect morphology of Schwann cells and activation of ERK1/2. MEK inhibitor PD98059 could, block this activation. It suggests that MEK/ERK signal pathway should be involved in Schwann cells proliferation promoted by PQQ.
