Effect of hirudin on random skin flap survival in rats.
- Author:
Ding-sheng LIN
1
;
Xin ZHENG
;
Zhi-jie LI
;
Yi-peng ZHANG
;
An-yuan WANG
;
Xue-guan XIE
;
Wei-yang GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fibroblast Growth Factor 2; metabolism; Graft Survival; drug effects; Hirudins; pharmacology; Male; Rats; Rats, Sprague-Dawley; Skin Transplantation; Surgical Flaps; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Plastic Surgery 2011;27(1):35-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Hirudin on random skin flap survival in rats.
METHODS24 SD rats were randomly divided into control group and experimental group. The "McFarlane flap (3 cm x 9 cm)" rat models were established on the rat dorsum. 3 ml Hirudin (30 ATU) was injected into the flap in the experimental group, while 3 ml saline in the control group. The injection was performed for 7 days. The flap survival area in the two groups was measured. The tissue samples were taken from proximal (I), middle (II) and distal (III) portions of flaps for histologic study. The VEGF and bFGF expression was also detected with immunohistochemistry method.
RESULTS7 days after operation, the flap survival rate was (69.52 +/- 3.23)% in the experimental group, while (50.36 +/- 2.37)% in control group, showing a significant difference between the two groups (P < 0. 01). In the middle portion, tissue edema and infiltration of neutrophils in experimental group was markedly slighter than that in control group. The VEGF and bFGF expression and neovascularization was enhanced markedly in experimental group.
CONCLUSIONSHirudin can increase the survival of random pattern skin flaps. It may increase the VEGF, bFGF expression through a series of complex regulatory pathway. Then flap neovascularization is promoted and the flap blood supply is increased.
