Related substances in purified alliin determined by HPLC-MS/MS.
- Author:
Yao-Zuo YUAN
1
;
Jie GU
;
Tai-Jun HANG
;
Jian CHEN
;
Zheng-Xing ZHANG
Author Information
1. Department of Pharmaceutical Analysis, Ministry of Education, China Pharmaceutical University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Cysteine;
analogs & derivatives;
analysis;
Tandem Mass Spectrometry;
methods
- From:
Acta Pharmaceutica Sinica
2007;42(6):639-642
- CountryChina
- Language:Chinese
-
Abstract:
To study the related substances in purified alliin, HPLC-MS/MS method carried out on a Phenomenex NH2 column was used for screen and identification of the related substances with full scan MS spectra determination of their [M + H] + ions and then the analyses of the retention time, product MS spectra and/or chemical reference standards. The full scan HPLC-MS chromatogram showed that there were seven major related substances in the purified alliin and their m/z of the [M + H] + ions with increasing retention were 116, 133, 147, 152, 175, 178 and 178, separately. And they were identified as proline, asparagine, glutamine, methiin, arginine, isoalliin and cycloalliin (both were isomers of alliin), respectively. The major related substances in purified alliin are amino acids, homologen and the isomers of alliin.
