Analysis of supercritical fluid extracts of Radix caulophylli with gas chromatography-mass spectrometry.
- Author:
Si-Cen WANG
1
;
Qin-Hua CHEN
;
Yao-Yuan WEI
;
Han-Wen LI
;
Lang-Chong HE
Author Information
1. School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. wangsc@mail.xjtu.edu.cn
- Publication Type:Journal Article
- MeSH:
Carbon Dioxide;
Caulophyllum;
chemistry;
Chromatography, Supercritical Fluid;
Gas Chromatography-Mass Spectrometry;
Linoleic Acid;
analysis;
Palmitic Acid;
analysis;
Plant Roots;
chemistry;
Plants, Medicinal;
chemistry
- From:
Acta Pharmaceutica Sinica
2007;42(5):525-528
- CountryChina
- Language:Chinese
-
Abstract:
To analyze the constituents in supercritical fluid CO2 extraction (SFE-CO2) of Radix caulophylli, the Radix caulophylli was extracted with SFE-CO2, and analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis with a DB-5MS capillary column (30 mm x 0.32 mm ID, 0.25 microm film thickness) was used. The inlet temperature was maintained at 280 degrees C. The column oven was held at 80 degrees C for 2 min, then programmed from 80 to 280 degrees C at 5 degrees C x min(-1) and, finally, held for 4 min. Helium at a constant flow rate of 2.0 mL x min(-1) was used as the carrier gas. The mass spectrometry conditions were as follows: ionization energy, 70 eV; ion source temperature, 200 degrees C. The mass selective detector was operated in the TIC mode (m/z was from 40 - 500). For the first time 49 peaks were separated and identified, the compounds were quantitatively determined by normalization method, and the identified compounds represent 97.44% of total GC peak areas. Viz, n-hexadecanoic acid (31.4%), (E, E) -9, 12-octadecadienoic acid (26.54%), (Z)-7-tetradecenal (9.4%), hexadecenoic acid (3.23%), 10-undecenal (3.22%), octadecanoic acid (2.25%), and caulophylline (1.76%) etc. The results will provide important foundation for understanding the constituents and further exploitation of Radix caulophylli.
