The entrapped efficiency of BSA liposome.
- Author:
Dong-Zhi HOU
1
;
Chang-Ke LIU
;
Qi-Neng PING
;
Xiao-Hui LIANG
Author Information
1. School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China. houdongzhi@sina.com
- Publication Type:Journal Article
- MeSH:
Colorimetry;
Drug Carriers;
Drug Compounding;
Electrophoresis, Gel, Two-Dimensional;
Liposomes;
Particle Size;
Rosaniline Dyes;
Serum Albumin, Bovine;
administration & dosage;
analysis;
Spectrophotometry, Ultraviolet
- From:
Acta Pharmaceutica Sinica
2007;42(5):545-549
- CountryChina
- Language:Chinese
-
Abstract:
BSA liposomes were prepared with approximately 100 nm mean particle size under rather gentle experiment conditions, and two-colorimetric coomassie brilliant blue protein was employed to measure the free drug in the entrapped efficiency (EE%) determination of BSA liposomes. Gel filtration was used to measure the EE%, and several Sephadex gels were examined by the separation of liposomes and free drug. To determine the free drug, three methods were compared on two-colorimetric UV spectrophotography, Bradford and two-colorimetric coomassie brilliant blue, separately. Two-colorimetric coomassie brilliant blue process increased the accuracy and improved the sensitivity of the assay about 20-fold comparing with the Bradford method. Two-colorimetric coomassie brilliant blue assay appeared to be more sensitive and showed broader dynamic range to measure the free BSA in the EE% determination of BSA liposome.
