Determination of mosapride in human plasma by high performance liquid chromatography tandem mass spectrometry.
- Author:
Feng QIN
1
;
Ling-Yun CHEN
;
Yuan-Yuan MA
;
Dong WANG
;
Juan LIU
;
Xiu-Mei LU
;
Fa-Mei LI
Author Information
1. College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Area Under Curve;
Benzamides;
administration & dosage;
blood;
pharmacokinetics;
Chromatography, High Pressure Liquid;
methods;
Gastrointestinal Agents;
administration & dosage;
blood;
pharmacokinetics;
Humans;
Male;
Morpholines;
administration & dosage;
blood;
pharmacokinetics;
Sensitivity and Specificity;
Serotonin Receptor Agonists;
administration & dosage;
blood;
pharmacokinetics;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry;
methods
- From:
Acta Pharmaceutica Sinica
2007;42(8):882-885
- CountryChina
- Language:Chinese
-
Abstract:
To develop a sensitive and specific high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the determination of mosapride in human plasma, mosapride and internal standard tamsulosin were extracted from plasma with liquid-liquid extraction, then separated on a Waters ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 microm ID) with gradient elution at flow-rate of 0.25 mL x min(-1). The mobile phase was water (containing 0.3% formic acid) and acetonitrile under gradient conditions. Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 422 --> m/z 198 and m/z 409 --> m/z 228 were used to quantify mosapride and the internal standard, respectively. The linear calibration curve was obtained in the concentration range of 0.17 - 68.00 ng x mL(-1). The lower limit of quantification was 0.17 ng x mL(-1). The inter- and intra-day precision (RSD) was less than 13%, and the accuracy (RE) was within +/- 6.3% calculated from QC samples. The method was used to determine the concentration of mosapride in plasma after a single oral dose of 5 mg mosapride citrate to 20 healthy male Chinese volunteers. The method has been proved to be selective, sensitive, rapid and suitable for pharmacokinetic study of mosapride.
