Effects of Ginkgo biloba extract on expressions of IL-1beta, TNF-alpha, and IL-10 in U937 foam cells.
- Author:
Ya-Bin JIAO
1
;
Yao-Cheng RUI
;
Peng-Yuan YANG
;
Tie-Jun LI
;
Yan QIU
Author Information
1. Department of Pharmacology, School of Pharmacy, The Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- MeSH:
Drugs, Chinese Herbal;
isolation & purification;
pharmacology;
Foam Cells;
metabolism;
Ginkgo biloba;
chemistry;
Humans;
Interleukin-10;
biosynthesis;
genetics;
Interleukin-1beta;
biosynthesis;
genetics;
Lipoproteins, LDL;
Plants, Medicinal;
chemistry;
RNA, Messenger;
metabolism;
Receptors, Interleukin-10;
biosynthesis;
genetics;
Tumor Necrosis Factor-alpha;
biosynthesis;
genetics;
U937 Cells
- From:
Acta Pharmaceutica Sinica
2007;42(9):930-934
- CountryChina
- Language:English
-
Abstract:
This study is to investigate the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in U937 foam cells and effects of Ginkgo biloba extract (GbE) on the cytokines. U937 cells were cultured with different concentrations of GbE (0.1, 1, and 10 microg x L(-1)), and stimulated by 100 mg x L(-1) oxidized low density lipoprotein (ox-LDL) for 24 h. The expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) in culture solution were detected by enzyme-linked immunosorbant assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that incubated with 100 mg x L(-1) ox-LDL for 24 h, the U937 cells became foam cells, the protein or mRNA expressions of IL-1beta, TNF-alpha, IL-10, and its receptor IL-10R in U937 foam cells were higher markedly than those in normal U937 cells. When the cells were pretreated with GbE (0.1, 1, and 10 microg x L(-1)), the increases of IL-1beta and TNF-alpha in U937 foam cells were remarkably inhibited, but IL-10 expression increased greatly. Especially when cells were pretreated with 10 microg x L(-1) GbE, the protein and mRNA expressions of IL-1beta and TNF-alpha were markedly lower than those in U937 foam cells. The protein expression of IL-10 and mRNA expressions of IL-10 and its receptor IL-10R were markedly higher than those in U937 foam cells. GbE inhibited production of pro-inflammatory cytokines IL-1beta and TNF-alpha, but up-regulated the production of anti-inflammatory cytokine IL-10 and its receptor IL-10R in U937 foam cells, which might be related with its anti-atherosclerotic actions.
