Protective effect of (-) clausenamide against neurotoxicity induced by okadaic acid and beta-amyloid peptide25-35.
- Author:
Jing ZHANG
1
;
Yong CHENG
;
Jun-Tian ZHANG
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Amyloid beta-Peptides;
antagonists & inhibitors;
toxicity;
Animals;
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Survival;
drug effects;
Cerebral Cortex;
cytology;
Clausena;
chemistry;
Drugs, Chinese Herbal;
isolation & purification;
pharmacology;
Female;
Hippocampus;
cytology;
Humans;
L-Lactate Dehydrogenase;
metabolism;
Lactams;
isolation & purification;
pharmacology;
Learning;
drug effects;
Lignans;
isolation & purification;
pharmacology;
Memory Disorders;
physiopathology;
Neuroblastoma;
metabolism;
pathology;
Neurons;
drug effects;
Neuroprotective Agents;
isolation & purification;
pharmacology;
Okadaic Acid;
antagonists & inhibitors;
toxicity;
Ovariectomy;
Peptide Fragments;
antagonists & inhibitors;
toxicity;
Plants, Medicinal;
chemistry;
Rats;
Rats, Sprague-Dawley
- From:
Acta Pharmaceutica Sinica
2007;42(9):935-942
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the protective effect of (-) clausenamide against the neurotoxicity of okadaic acid in SH-SY5Y cell line, and injection beta-amyloid peptide25-35 (Abeta25-35) to the cerebral ventricle in ovariectomy (OVX) rats. MTT assay, LDH assay, and Hoechst 33258 staining were used to detect the effect of (-) clausenamide on the toxicity of okadaic acid in SH-SY5Y cell line. The animal model was induced by ovariectomized and injection of Abeta25-35 in the cerebroventricle of rats. The effect of (-) clausenamide on learning and memory deficiency was observed by step-through test. Electron microscope, Nissl body staining, and HE staining were used to examine the morphological changes in hippocampus and cerebral cortex neurons. Pretreatment of (-) clausenamide and LiCl decreased the rate of cell death from MTT, LDH release, and apoptosis from Hoechst 33258 staining in SH-SY5Y cell line. The step-through tests showed (-) clausenamide could improve the ability of learning and memory. The Nissl body staining and HE staining experiments also showed the neuroprotective effects of (-) clausenamide on the neurons of hippocampus and cerebral cortex. (-) Clausenamide has the protective effects against the neurotoxicity induced by okadaic acid and Abeta25-35.
