Determination of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae by HPLC-UV-ELSD.
- Author:
Dong ZHANG
1
;
Lan YANG
;
Li-Xin YANG
;
Man-Yuan WANG
;
You-You TU
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- MeSH:
Artemisia annua;
chemistry;
Artemisinins;
analysis;
Chromatography, High Pressure Liquid;
methods;
Light;
Plant Components, Aerial;
chemistry;
Plants, Medicinal;
chemistry;
Reproducibility of Results;
Scattering, Radiation;
Sensitivity and Specificity;
Spectrophotometry, Ultraviolet;
methods
- From:
Acta Pharmaceutica Sinica
2007;42(9):978-981
- CountryChina
- Language:Chinese
-
Abstract:
To establish an HPLC-UV-ELSD method for the determination of the content of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae. The analytical column was Nucleodur RP-C18 (250 mm x 4.6 mm, 5 microm ID). The mobile phase was acetonirile-0.1% acetic acid (50: 50) and the flow rate was 1.0 mL x min(-1) with a UV detector for artemisinin, the detection wavelength at 209 nm, and the evaporative light-scattering detector (ELSD) for arteannuin B and artemisinic acid, the drift tube temperature: 50 degrees C, the nitrogen flow rate 30 psi and the gain was 50. The resolution of artemisinin, arteannuin B and artemisinic acid was good. The linear calibration curves were obtained over the range of 0.52 - 2.6 microg for artemisinin (r = 0.999 4, n = 5), 0.022 - 4.4 microg for artemisinin B (r = 0.999 9, n = 5) and 0.203 - 8.12 microg for artemisinic acid (r = 0.999 8, n = 5), separately. The mean recoveries of the three compounds were 99.45%, 102.37% and 101.10% with RSD of 2.3%, 1.7% and 0.79%, respectively. This method is simple, rapid, accurate and suitable for the determination of the content of the three compounds in the herbs.
