Effects of drugs known to trigger psoriasis on HaCaT keratinocytes.
- Author:
Jian-ping CEN
1
;
Ke-jian ZHU
;
Na JIN
;
Ai-hua LIN
;
Hao CHENG
Author Information
1. Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
- Publication Type:Journal Article
- MeSH:
Adrenergic beta-Antagonists;
adverse effects;
Antimalarials;
adverse effects;
Cells, Cultured;
Chloroquine;
adverse effects;
analogs & derivatives;
Humans;
Interleukin-6;
biosynthesis;
genetics;
Interleukin-8;
biosynthesis;
genetics;
Keratinocytes;
drug effects;
metabolism;
Lithium Carbonate;
adverse effects;
Propranolol;
adverse effects;
Psoriasis;
metabolism;
RNA, Messenger;
metabolism;
Tumor Necrosis Factor-alpha;
pharmacology
- From:
Acta Pharmaceutica Sinica
2007;42(10):1041-1044
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether lithium carbonate, propranolol or chloroquine aggravate psoriasis through influencing cytokines of the psoriatic cytokine network, HaCaT keratinocytes were stimulated with TNF-a after treatment with these drugs. Protein secretion of a set of multiple different cytokines and growth factors in culture supernatants were measured by using a cytokine antibody array technology. Expression of IL-8 and IL-6 mRNA was determined by real-time PCR. In culture supernatants of TNF-alpha-stimulated HaCaT cells, production of IL-6 and TNF-alpha could be enhanced by lithium carbonate; production of IL-6 and a panel of cytokines and growth factors could be enhanced by propranolol hydrochloride; and IL-6 was up-regulated by chloroquine diphosphate as well. Real-time PCR analysis showed a significantly dose-dependent increase of IL-8 and IL-6 mRNA expression in HaCaT cells stimulated with TNF-a as compared to cells without TNF-alpha-stimulation, the mRNA expression of IL-8 was higher than that of IL-6 with the same concentration of TNF-alpha (P < 0.01). Compared with HaCaT cells cultured with medium alone, propranolol hydrochloride at the concentration of 1 x 10(-6) mol x L(-1) could stimulate HaCaT cells to express higher level of IL-6 mRNA (P < 0.05). The drugs investigated show a modulatory effect on certain cytokines and growth factors which are able to modulate inflammatory type of immune reaction present in psoriatic lesions.
