Expression and cellular localization of interleukin-8 mRNA and protein in the area of xenogenic bone implant.
- Author:
Hu XU
1
;
Yunyu HU
;
Shifeng CAO
Author Information
1. Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
- Publication Type:Journal Article
- From:
Chinese Journal of Traumatology
1999;2(1):3-7
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE: To observe the expression and cellular localization of interleukin-8 (IL-8) mRNA and protein in the area of xenogenic bone implant. METHODS: The bovine cancellous bone granules were implanted into the thigh muscles of mice. The samples were taken 4, 7, 14 and 21 days after implantation. IL-8mRNA and protein in the site of implant were assayed by in situ hybridization and immunocytochemical techniques. RESULTS: The expression of IL-8mRNA and protein were observed in all specimens 4, 7, 14 and 21 days after implantation. IL-8mRNA was expressed mainly by the neutrophils, monocytes, macrophages and fibroblasts at 7th day post-implantation. Some mesenchymal cells, multinucleated giant cells, vascular endothelial cells and smooth muscle cells also expressed IL-8mRNA in the area of xenogenic bone implant at 14th and 21st days. Immunocytochemical studies demonstrated the same results as that of in situ hybridization. CONCLUSIONS: Many different kinds of cells express IL-8mRNA and secret IL-8 in the area of xenogenic bone implant, suggesting that IL-8 may play an important role in local immunity of xenogenic bone graft.
