Osteogenic potential of rabbit dermal fibroblasts cultured in vitro: a scanning electron microscopic study.
- Author:
Benfu CHAI
1
;
Xueming TANG
;
Hui LI
Author Information
1. Shanghai Institute of Traumatology and Orthopaedics, Shanghai 200025, China.
- Publication Type:Journal Article
- From:
Chinese Journal of Traumatology
1999;2(2):75-78
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE: To investigate further into the osteogenic potential of rabbit dermal fibroblasts with scanning electron microscope. METHODS: Split-thickness rabbit skin was processed into small chips that were cultured in vitro and then subjected to scanning electron microscopic study. RESULTS: The fibroblasts swam out from the skin chips and they increased in number rapidly and became confluent. The cells exhibited squamous configuration, possessing arboreal bifurcation and forming multi-layer structure. The fibroblasts then excreted numerous minute granules, heaping up on and around the cells. Thenceforth emerged on the cell surface fine needle-like crystals, that agglomerated with the granules to form nodules. The fibroblasts orientated themselves in a radiating pattern around the large nodules. Neighboring nodules could be linked up into trabecular structures. CONCLUSIONS: Sequence of events of new bone formation by rabbit dermal fibroblasts cultured in vitro is fully depicted and confirmed.
