Effect of bactericidal/permeability-increasing protein on sepsis induced by intra-abdominal infection in rats.
- Author:
Jianxin JIANG
1
;
Guoqi XIE
;
Dawei LIU
;
Peifang ZHU
;
Zhengguo WANG
;
Yani HE
;
Jihong ZHOU
;
Hong XU
Author Information
1. Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
- Publication Type:Journal Article
- From:
Chinese Journal of Traumatology
1999;2(2):84-86
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE: To investigate the effect and mechanism of bactericidal/permeability-increasing protein (BPI) on sepsis induced by intra-abdominal infection in rats. METHODS: Cecal ligation and puncture (CLP) was made on 20 rats with sepsis induced by intra-abdominal infection. BPI or equal volume of physiological saline (PS) was intra-abdominally given immediately and 12 h after CLP, respectively (2.5 mg/kg of BPI each time). Plasma endotoxin levels were determined with limulus amebocyte chromogenic assay. RESULTS: (1) The survival time in BPI group was significantly higher than that in PS group. (2) The values of the mean arterial pressure (MAP), the left ventricular systolic pressure (LVSP), the isovolumic ventricular pressure (IP), and the maximal change of left intraventricular pressure (+/-dp/dtmax) in BPI group, although decreasing, were markedly higher than those in PS group. (3) Plasma glutamic-pyruvic transaminase (GPT) and urea nitrogen levels in BPI group, though increasing, were obviously lower than those in PS group. (4) There was no significant change of plasma endotoxin levels in BPI group, while plasma endotoxin levels markedly increased in PS group. CONCLUSIONS: BPI has obvious protective effect on sepsis induced by intra-abdominal infection, which might be related to its neutralization of endotoxin.
