Up-regulation of TIMP-2 expression promotes SHI-1 leukemic cells proliferation and infiltration in immunodeficiency mice.
- Author:
Zhenjiang LI
1
;
Zixing CHEN
2
;
Jiannong CEN
3
;
Jun HE
3
;
Qiaocheng QIU
3
;
Yongquan XUE
3
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Animals; Cell Line; Cell Proliferation; physiology; Humans; Leukemic Infiltration; physiopathology; Male; Mesenchymal Stromal Cells; metabolism; physiology; Mice; Mice, Inbred BALB C; Tissue Inhibitor of Metalloproteinase-2; genetics; metabolism
- From: Chinese Medical Journal 2014;127(24):4243-4249
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDMMPs and TIMPs play important roles in tumor angiogenesis and invasion. Studies have shown that TIMP-2 has two roles in tumor invasion. However, its role in leukemic infiltration has not been well investigated. This study explored the roles of TIMP-2 in extramedullary infiltration of acute monocytic leukemic SHI-1 cells both in vitro and in vitro.
METHODSA retroviral vector carrying the human TIMP-2 cDNA was constructed and transfected into the monocytic leukemic cell line SHI-1. The expression of TIMP-2 in the positive clones was determined. The proliferation of SHI-1 cells was examined by MTT assay. Trans-Matrigel invasion assays were used to investigate the infiltration ability in vitro. SHI-1 cells were intravenously injected into pre-treated nu/nu mice to investigate the infiltration ability feature in vitro.
RESULTSThe expression of TIMP-2 on the cell membrane was significantly elevated in SHI-1/TIMP-2 cells. Over-expression of TIMP-2 promoted the cells proliferation and the invasions in vitro. The SHI-1/TIMP-2 cells demonstrated higher infiltration ability when intravenously injected into nu/nu mice.
CONCLUSIONOver-expression of TIMP-2, especially on the cell membrane, may play important roles in promoting the proliferation and infiltration of SHI-1 leukemic cells.
