Prenatal exposure to lipopolysaccharide results in vascular renin-angiotensin system dysfunction in offspring rats
10.3760/cma.j.issn.0253-3758.2011.09.017
- VernacularTitle:孕期脂多糖刺激对子代大鼠血管肾素血管紧张素系统的影响
- Author:
Xiao-Yan HE
1
;
You-Cai DENG
;
Shu-Hui LI
;
Yan-Ling WEI
;
Li-Yun HAO
;
Xing-Xing ZHANG
;
Xiao-Hui LI
Author Information
1. 第三军医大学
- Keywords:
Hypertension;
Inflammation;
Blood vessels;
Renin-angiotensin system
- From:
Chinese Journal of Cardiology
2011;39(9):853-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of prenatal exposure to lipopolysaccharide on reninangiotensin system of offspring rats Methods Six pregnant SD rats were randomly divided into 2 groups.The rats in the lipopolysaccharide (LPS) group were treated with LPS 0. 79 mg/kg (I. P. ) on the 8th, 10th and 12th day of gestation, and rats in the control group were treated with saline at the same time points. The blood pressure of offspring rats was measured by the tail cuff method. Protein expression of Angiotensin Ⅱ (Ang Ⅱ ) in thoracic aorta vessel was determined by immunohistochemisry. Protein expressions of Ang Ⅱtype 1 and type 2 receptor in thoracic aorta vessel were detected by Western blot. Results Blood pressure of 12-week-old offspring rats of LPS group was significantly higher than that of 12-week-old offspring rats of control group( P ＜ 0. 01 ). The protein expression of Ang Ⅱ and Ang Ⅱ type 1 receptor in thoracic aorta vessel were significantly higher while protein expression of Ang Ⅱ type 2 receptor was lower in 15-week-old offspring rats of LPS group than in control group, resulting in a significant increase in the ratio of Ang Ⅱtype 1 receptor/Ang Ⅱ type 2 receptor in the aorta at 15-week-old of offspring rats than in 15-week-old offspring rats of control group( P ＜ 0. 01 ). Conclusion Prenatal lipopolysaccharide exposure results vascular renin-angiotensin system dysfunction, which my play an important role on the pathogenesis of hypertension development in offspring rats.
