Establishment of a cyanotic congenital heart defect porcine model with decreased pulmonary blood flow
10.3760/cma.j.issn.0253-3758.2011.10.007
- VernacularTitle:肺血减少型先天性心脏病幼猪动物模型的立及其未成熟肺血管的形态学变化
- Author:
Kang-Wu WANG
1
;
Xue-Gang LIU
;
Chao SHI
;
Ge LIU
;
Lei ZHANG
;
An-Sheng WANG
;
Chao SONG
Author Information
1. 蚌埠医学院附属第一医院
- Keywords:
Heart defects,congenital;
Models,animal;
Diminutive pulmonary blood
- From:
Chinese Journal of Cardiology
2011;39(10):905-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a porcine model of congenital heart disease with decreased pulmonary blood to explore the morphological changes of immature pulmonary vascular vessels.Methods Twenty piglets (one to two-month-old ) were randomly divided into three groups:sham-operated group (group S,n =6),small incisions on the right chest,produced a transient reduction in pulmonary blood;Operation group 1 ( group T1,n =7),small incisions on the right chest,producing artificial atrial defect with self-made dilator and simultaneous banding pulmonary artery to generate a systolic pressure gradient between 20 - 30 mm Hg(1 m Hg =0.133 kPa) ; Operation group 2(group T2,n =7):operation procedure was similar as group T1 with systolic pressure gradient between 30 -50 mm Hg.Lung tissue from right middle lobe ( 1.0 cm × 0.8 cm × 0.8 cm) was taken immediately after thoracotomy,at the end of surgery and at 2 months after operation and stained by Weigert (elastic fiber) and van Gieson (collagen) methods to observe the morphological changes.Results Five animals survived in Group S,6 animals survived in group T1 and 5 animals survived in group T2.The inside diameter of pulmonary arterioles after thoracotomy and at the end of surgery was similar among the three groups (P ＞0.05).At 2 months after operation,the inside diameter of pulmonary artery was significantly higher in group T1 and T2 than in group S (all P ＜ 0.05 )while the number of pulmonary small artery per square centimeter (APSC) of group T1 and T2 was significantly lower than that of group S ( all P ＜ 0.05).Tunica media of pulmonary artery was thinner and vascular lumen was larger in group T1 and T2 compared to those of group S.Conclusion In this piglets model with reduced pulmonary blood,the pulmonary arterioles underwent dysplastic changes.Thus,pulmonary blood flow is an important determinant for the physiological development of pulmonary artery.
