Stents coated with sirolimous and anti-CD34 antibody can optimize the performance of sirolimouseluting stents
10.3760/cma.j.issn.0253-3758.2011.11.006
- VernacularTitle:抗CD34抗体优化雷帕霉素洗脱支架疗效的实验研究
- Author:
Xian-Tao SONG
1
;
Hua-Gang ZHU
;
Xing-Sheng YANG
;
Fei YUAN
;
Shu-Zheng L(U)
Author Information
1. 首都医科大学附属北京安贞医院
- Keywords:
Stcnts;
Antibodies;
Treatment outcome
- From:
Chinese Journal of Cardiology
2011;39(11):997-1004
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of the stents coated with sirolimous and anti-CD34 antibody on the short-term re-endothelialization and the long-term restenosis in Chinese Minipigs.Methods Three different types of stents [ bare-metal stent ( BMS ),sirolimous-eluting stent ( SES ) and anti-CD34 antibody and sirolimous-coated stent (ASES) ] were randomly implanted in the coronary arteries of 22Chinese Minipigs.At two weeks after stenting,coronary angiography and optical coherence tomography (OCT) were performed in 10 experimental animals.At three months after stenting,coronary angiography and OCT were performed in the remaining 12 experimental animals.Histopathologic examination was performed on the coronary artery segments containing stent after the animals were executed.Results (1) No in-stent thrombosis and parietal thrombus were found by coronary angiography,OCT and histopathologic examination at two weeks post stenting.OCT analysis showed that the covered ratio of stent struts by neointima in ASES group was higher than in SES group [ ( 55.56 + 35.27 ) ％ vs.(41.82 +23.28 ) ％,P ＜ 0.05 ].The mean thickness of neointima in ASES group was significantly higher than in SES group [(89.0+5.0)1.μn vs.(32.0 +4.9) μm,P＜O.O1] and BMS group [(89.0 +5.0) μm vs.(44.0 ± 7.2) μm,P ＜ 0.01].Histopathologic and scanning electron microscopy examinations demonstrated that the covering level and quality of stent struts by neointima in BMS and ASES group were both better than in SES group.(2) At three months follow-up,quantitative coronary angiography analysis found that late instent lumen loss in ASES group was significantly lower than in BMS group [ (0.18 ±0.06)mm vs.(0.35 ±0.06)mm,P ＜ 0.05].OCT analysis showed that the percent neointimal hyperplasia in ASES and SES group was significantly lower than in BMS group [ ( 34.75 + 2.64) ％ and ( 35.63 + 2.07 ) ％ vs.(48.28 ±3.25) ％,both P ＜ 0.01 ].Histopathologic analysis demonstrated that the percent areal restenosis of ASES and SES group were both significantly lower than that of BMS group [ (28.65 + 5.64)％ and (29.33 ±6.07)％ vs.(46.18 +8.25)％,both P ＜0.05].Conclusion The stents coated with anti-CD34 antibody and sirolimous can attenuate the inhibitory effect of sirolimous on the re-endothelialization at two weeks after stenting and the anti-hyperplasia effect of sirolimous at three months after stenting.
