Resolvin E1 protects against ox-LDL-induced injury on vascular endothelial cells
10.3760/cma.j.issn.0253-3758.2011.11.014
- VernacularTitle:消退素E1对氧化型低密度脂蛋白诱导的血管内皮细胞损伤的保护作用
- Author:
Ya-Feng CHEN
1
;
Hong JIANG
;
Xia GONG
;
Jing-Yuan WAN
Author Information
1. 荆门市第一人民医院
- Keywords:
Endothelial cells;
Apoptosis;
Lipoproteins,LDL;
Resolvin E1
- From:
Chinese Journal of Cardiology
2011;39(11):1039-1043
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether Resolvin E1 ( RvE1 ) could protect against ox-LDL-induced injury on human vein vascular endothelial cells and reveal related molecular mechanisms.Methods Human vein vascular endothelial cells were randomly assigned to six groups,which were treated with saline,RvE1,wortmanin,ox-LDL,ox-LDL and RvE1,ox-LDL and RvE1 and wortmanin,respectively.After 48 h,survival rates were determined by MTT,apoptosis rate of cells were determined by flow cytometry,TNF-αt contents were assayed by ELISA,caspase 3 and 9 activities were measured by microplate reader,and the expression of p-AKT and LOX-1 were determined by Western blot.Result Compared with normal saline group,survival rate was markedly decreased and apoptosis rate,TNF-α content,caspase 3 and 9 activities,and the expression of LOX-1 were significantly increased in ox-LDL group ( P ＜ 0.01 ).Survival rate was significantly increased and apoptosis rate,TNF-α content,caspase 3 and 9 activities,and the expression of LOX-1 were significantly decreased in ox-LDL + RyE1 group compared to ox-LDL group (P ＜ 0.01 ),these beneficial effects of RvE1 could be blocked by PI3K inhibitor wortmanin ( P ＜ O.05 ).Conclusion The present data showed that RvE1 could effectively protect against ox-LDL-induced endothelial cell injury,which might be mediated by PI3K-AKT signaling pathway.
