Role of caveolin-I on membrane estrogen receptor mediated proliferation of endothelial progenitor cells
10.3760/cma.j.issn.0253-3758.2011.11.015
- VernacularTitle:小窝蛋白-1在膜雌激素受体介导的内皮祖细胞增殖中的作用
- Author:
Fei-Xue HU
1
;
Ting-Huai WANG
;
Zhi TAN
Author Information
1. 北京大学深圳医院
- Keywords:
Endothelium,vascular;
Stem cells;
Caveolin-1;
Estrogens;
Receptors,estrogen
- From:
Chinese Journal of Cardiology
2011;39(11):1044-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential role of caveolin-1 ( CAV-1 ) on membrane estrogen receptor (mER) mediated proliferation of endothelial progenitor cells (EPCs).Methods Bone marrow (BM) -derived EPCs were cultured.The proliferation of EPCs induced by estradiol ( E2 ) -BSA in the absence or presence of ICI 182,780 (a pure ER inhibitor),MβCD and CAV-1 siRNA was determined by [3H]-thymidine incorporation.The expression of CAV-1 was detected by Western blot.Results Proliferation of EPC peaked after 10-8 mol/L E2-BSA culture for 24 h (87.5％ increase vs.control),and this effect could be inhibited by estrogen receptor blocker ICI 182,780,indicating that mER-initiated membrane signaling pathways was involved in the proliferation effect of estrogen on EPC.Both cholesterol depletion and CAV-1 siRNA significantly attenuated E2-BSA induced [3H ]-thymidine incorporation.Western blot result confirmed that cholesterol depletion or CAV-1 siRNA significantly decreased CAV-1 protein expression ( - 18.6％ or -41.2％ vs.10-8 mol/L E2-BSA alone).Conclusion Our results suggested that estradiol promoted EPC proliferation through activating CAV-1 pathway.
