PECAM-1 and E-selectin expression in vulnerable plague and their relationships to myocardial Leu125Vai polymorphism of PECAM-1 and Ser128Arg polymorphism of E-selectin in patients with acute coronary syndrome
10.3760/cma.j.issn.0253-3758.2011.12.007
- VernacularTitle:急性冠状动脉综合征易损斑块的血小板内皮细胞黏附分子1、内皮细胞选择素抗体与心肌中相关基因多态性的研究
- Author:
Fang FANG
1
;
Wei ZHANG
;
Li YANG
;
Zheng WANG
;
Dong-Ge LIU
Author Information
1. 卫生部北京医院
- Keywords:
Coronary atherosclerosis;
E-selectin;
Antigens,CD31;
Polymorphism,single nucleotide;
Autopsy
- From:
Chinese Journal of Cardiology
2011;39(12):1110-1116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of PECAM-1 and E-selectin in the vulnerable plagues and their relationships to myocardial Leu125Val polymorphism of PECAM-1 and Ser128Arg polymorphism of E-selectin in autopsied samples of patients with acute coronary syndrome (ACS).Methods We detected the expressions of PECAM-1 and E-selectin in the vulnerable plaques by immunohistochemistry on 50 autopsy samples of patients with ACS,30 autopsy samples from non-cardiac disease patients served as control.Genetic Leu125Val polymorphism of PECAM-1 was detected by PCR-SSCP in myocardial paraffin blocks of 37 ACS cases and 43 control cases,and Ser128Arg polymorphism of E-selectin was detected by PCR-RFLP in myocardial paraffin blocks of 39 ACS cases and 43 control cases,respectively.Results Immunohistochemical features:( 1 ) the incidence of positive expression in the intima of coronary artery of PECAM-1 [76.0％ (38/50) vs.26.7％ (8/30) ] and E-selectin [26.0％ (13/50) vs.0] was significantly higher in ACS group than in control group (all P ＜0.01 ).(2) Expressions of PECAM-1 [58.0％ (29/50)vs.28.0％ ( 14/50 ) ] and E-selectin [ 22.0％ ( 11/50 ) vs.12.0％ ( 6/50 ) ] were significantly higher at neovascular endothelial cells in plaques than expressions at coronary arterial endothelial cells in ACS group (all P ＜0.01 ).(3) In 41 plaques with inflammatory infiltration,the expression rates of PECAM-1 and E-selectin in inflammatory cell density of ＜ 10,10 -30 and ＞30/HPF were 33.3％,68.2％,92.3％ and 16.7％,31.8％ and 23.1％,respectively.Genotype detection results:There is significant difference in frequencies of allele in Leu125Val polymorphism ( P ＜ 0.05 ),but the genotype distributional frequencies were similar ( P ＞ 0.05 ) between ACS group and control group.There are significant differences in frequencies of allele and genotype in Ser128Arg of E-selectin polymorphism between ACS group and control group ( all P ＜ 0.05 ).Conclusions The immunohistochemical expressions of PECAM-1 and E-selectin were significantly increased at intima in vulnerable plaques of ACS group,especially in neovascular endothelial cells,and positively correlated with inflammatory cell density,suggesting that PECAM-1 and E-selectin might play an important role in inflammatory reaction and development of vulnerable plaque.E-selectin Ser128Arg polymorphism is associated with ACS,and it might be a risk factor for ACS.
