Alteration of interleukin-17/interferon-γ double positive cells in mice with Coxsackie virus induced myocarditis

VernacularTitle:病毒性心肌炎小鼠白细胞介素17和γ干扰素双阳性细胞的变化
Author: Fan YANG ¹ ; Wei-Feng WU ; Yu-Luan YAN ; Yu PANG ; Qing KONG
Author Information

1. 广西医科大学第一附属医院
Keywords: Myocarditis; Interleukins; Interferon type Ⅱ; Lymphocytes
From: Chinese Journal of Cardiology 2011;39(12):1135-1139
CountryChina
Language:Chinese
Abstract: Objective To observe the alteration of interleukin-17 and interferon-γ double positive cells(IL-17 +/IFN-γ cells)in mice with coxsackie virus B3(CVB3)induced acute viral myocarditis(VMC).Methods VMC was induced in male Balb/c mice by peritoneal injection of CVB3.Control mice received PBS injection.At 0,1,2,3,4 and 6 weeks after injection,pathological scores were determined on hematoxylin-eosin stained heart sections and flow cytometric analysis was performed to evaluate the percent of IL-17+/IFN-γ+ cells among CD4+ T cells.Results Compared to control mice,the pathological scores of VMC mice were higher on CVB3 infection week 1(1.8 ± 0.5),peaked on week 2(2.8 ± 0.5)and declined thereafter.However,the proportion of IL-17 +/IFN-γ+ cells remained steadily at a low level throughout the observation period and was similar between VMC and control mice.Conclusions Our data shows that IL-17 +/IFN-γ+ cells might not be involved in the inflammation process of CVB3 induced VMC mice model.