Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry.
- Author:
Xiao-hua ZHAO
1
;
Bo SONG
;
Da-fang ZHONG
;
Shu-qiu ZHANG
;
Xiao-yan CHEN
Author Information
1. Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Chromatography, Liquid;
methods;
Glipizide;
administration & dosage;
blood;
pharmacokinetics;
Humans;
Hypoglycemic Agents;
administration & dosage;
blood;
pharmacokinetics;
Male;
Metformin;
administration & dosage;
blood;
pharmacokinetics;
Sensitivity and Specificity;
Tandem Mass Spectrometry;
methods;
Young Adult
- From:
Acta Pharmaceutica Sinica
2007;42(10):1087-1091
- CountryChina
- Language:Chinese
-
Abstract:
To develop a sensitive and rapid liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for simultaneous quantitation of metformin and glipizide in human plasma, metformin, glipizide and internal standard diphenhydramine were separated from plasma by protein precipitation with acetonitrile (containing 0.3% formic acid), then chromatographed by using a Zorbax Extend C18 column. The mobile phase consisted of acetonitrile-water-formic acid (70:30: 0.3, v/v/v), at a flow rate of 0.50 mL x min(-1). A tandem mass spectrometer equipped with atmospheric pressure chemical ionization source was used as detector and operated in the positive ion mode. Selected reaction monitoring (SRM) using the precursor/production combinations of m/z 130-->m/z 60, m/z 446-->m/z 321 and m/z 256-->m/z 167 were used to quantify metformin, glipizide and diphenhydramine, respectively. The linear concentration ranges of the calibration curves for metformin and glipizide were 2.00 - 2000 ng x mL(-1) and 1.00 - 1000 ng x mL(-1), respectively. The lower limits of quantitation of metformin and glipizide were 2.00 ng x mL(-1) and 1.00 ng x mL(-1), respectively. The method proved to be sensitive, simple and rapid, and suitable for clinical investigation of compound preparation containing metformin and glipizide.
