Isolation and activity of an alpha-amylase inhibitor from white kidney beans.
- Author:
Xiao-qi ZHANG
1
;
Ming-yan YANG
;
Yu MA
;
Jia TIAN
;
Ji-Rong SONG
Author Information
1. Shaanxi Microbiology Institute, Xi' an 710043, China. zhangxq282@163.com
- Publication Type:Journal Article
- MeSH:
Alloxan;
Amino Acids;
analysis;
Animals;
Blood Glucose;
metabolism;
Diabetes Mellitus, Experimental;
blood;
chemically induced;
Female;
Glycoproteins;
chemistry;
isolation & purification;
pharmacology;
Molecular Weight;
Monosaccharides;
analysis;
Phaseolus;
chemistry;
Plant Lectins;
chemistry;
isolation & purification;
pharmacology;
Plants, Medicinal;
chemistry;
Rats;
Rats, Sprague-Dawley;
Vegetable Proteins;
analysis;
alpha-Amylases;
antagonists & inhibitors
- From:
Acta Pharmaceutica Sinica
2007;42(12):1282-1287
- CountryChina
- Language:English
-
Abstract:
An alpha-amylase inhibitor (alpha-AI) was isolated from white kidney beans (Phaseolus vulgaris L) by ethanol fractional precipitation, ion exchange chromatography and gel filtration column chromatography. It was a homogeneity glycoprotein demonstrated by SDS-PAGE and gel filtration on CL-6B. The glycoprotein contained 88.2% protein and was rich in aspartic acid, glutamic acid, leucine, threonine and serine. The carbohydrate moiety was consisted of Man, Glc, Gal and Xyl in a mole ratio of 2.42: 1.50: 1.52: 1.00. The glycan and the core protein backbone was connected by O-linkage as determined by beta-elimination reaction. The continuous oral administration of the alpha-AI (150 mg x kg(-1) x d(-1)) for 7 days can lower fasting blood glucose and 300 mg x kg(-1) x d(-1) alpha-AI for 7 days can improve the sugar tolerance on alloxan-dependent diabetic model rats. The result showed the alpha-AI obtained from white kidney beans had good hypoglycemic effect on alloxan induced diabetic rats and may have high potential pharmaceutical value as a regulative digestive-starch degradation in patients suffering from diabetes.
