Analysis of aristolochic acid A from the aerial and underground parts of Asarum by UPLC-UV.
- Author:
Yan XUE
1
;
Xiang-Hui TONG
;
Feng WANG
;
Wei-Guo ZHAO
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. yanxue@imm.ac.cn
- Publication Type:Journal Article
- MeSH:
Aristolochic Acids;
analysis;
Asarum;
chemistry;
Chromatography, High Pressure Liquid;
methods;
Drug Stability;
Plant Components, Aerial;
chemistry;
Plant Roots;
chemistry;
Plants, Medicinal;
chemistry;
Reproducibility of Results;
Sensitivity and Specificity;
Spectrophotometry, Ultraviolet;
methods
- From:
Acta Pharmaceutica Sinica
2008;43(2):221-223
- CountryChina
- Language:Chinese
-
Abstract:
To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC HSST3 column (50 mm x 2.1 mm ID, 1.8 microm) was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL min(-1), the detection wavelength was 251 nm, and the column temperature was 40 degrees C. The calibration curve was linear in the range of 18 - 1450 ng of aristolochic acid A with correlation coefficient of 0. 9999 and the minimum detective concentration was 18.25 ng mL (-1). The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.
