Radiosensitivity of nasopharyngeal carcinoma in relation to cell cycle synchronization effect of tumor necrosis factor alpha.
- Author:
Jian YUAN
1
;
Ning SUN
;
Li-jing WANG
;
Wei TIAN
;
Xiao-ming ZHOU
;
Dong-bo CHEN
;
Hong-biao GU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Cycle; drug effects; radiation effects; Cell Line, Tumor; Humans; Mice; Mice, Nude; Nasopharyngeal Neoplasms; drug therapy; pathology; radiotherapy; Radiation-Sensitizing Agents; pharmacology; Tumor Necrosis Factor-alpha; pharmacology; Xenograft Model Antitumor Assays
- From: Journal of Southern Medical University 2007;27(3):374-376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of tumor necrosis factor alpha (TNFalpha) on radiosensitivity of nasopharyngeal carcinoma (NPC) in relation to TNFalpha-induced cell cycle synchronization.
METHODSThe radio-resistance of a NPC cell line subclone CNE-2Z-S1 was verified by in vivo experiments and flow cytometry was performed to evaluate cell cycle synchronization in TNFalpha-treated CNE-2Z-S1 cells. The radiosensitivity of the cell synchronized CNE-2Z-S1 cells was determined by clone formation in vitro and in vivo experiment in nude mice.
RESULTSTNFalpha was capable of inducing cell cycle arrest and synchronization of CNE-2Z-S1 cells. Pretreatment with TNFalpha remarkably enhanced the radiosensitivity of CNE-2Z-S1 in vitro, and in vivo experiments with nude mice also suggested the role of TNFalpha in enhancing the radiosensitivity of NPC.
CONCLUSIONTNFalpha can enhance the radiosensitivity of NPC cells by inducing cell cycle synchronization.
