Effect of platelet CD42a modification by mPEG-SPA with different molecular masses.
- Author:
Yin-ze ZHANG
1
;
Wen XIONG
;
Zhen LI
;
Chao-peng SHAO
;
Tian-jun LI
;
Feng ZHAO
;
Bao-cheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Blood Platelets; chemistry; Humans; Molecular Weight; Platelet Glycoprotein GPIb-IX Complex; chemistry; Polyethylene Glycols; chemistry; Succinimides; chemistry
- From: Journal of Southern Medical University 2007;27(3):392-393
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect platelet antigen modification by mPEG-SPA with different molecular masses.
METHODSPlatelet CD42a was modified by 5 kD and 20 kD mPEG-SPA, respectively, and the fluorescence intensity of CD42a was detect by flow cytometry and the three-dimensional structure of CD42a simulated to analyze the distribution of lysine in CD42a molecule.
RESULTSAfter platelet CD42a modification by 5 kD and 20 kD mPEG-SPA, the fluorescence intensity of CD42a decreased sharply by 85.54% and 88.65%, respectively, and multiple lysine regions were identified on the surface of CD42a molecule.
CONCLUSIONBoth 5 kD and 20 kD mPEG-SPA allow useful modification of platelet CD42a, but 20 kD mPEG-SPA is more advantageous than 5 kD mPEG-SPA.
