Expression of CCL20 and CXCR4 in epidermal condyloma acuminatum lesions.
- Author:
Jin-yun FENG
1
;
Zhen-hui PENG
;
Xiao-ping TANG
;
Mei-ju WANG
;
Yu-ping LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Blotting, Western; Chemokine CCL20; genetics; metabolism; Condylomata Acuminata; genetics; metabolism; Down-Regulation; Epidermis; metabolism; pathology; Gene Expression Regulation; Humans; Oligonucleotide Array Sequence Analysis; RNA, Messenger; genetics; metabolism; Receptors, CXCR4; genetics; metabolism; Young Adult
- From: Journal of Southern Medical University 2007;27(4):418-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum (CA) and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis.
METHODSGene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting.
RESULTSMicroarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis.
CONCLUSIONThe protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.
