Comparative proteomic analysis of the prefrontal cortex between normal and heroin-addicted rats.
- Author:
Ping-ming QIU
1
;
Hui-jun WANG
;
Chao LIU
;
Xue-feng LI
;
Xiao-hui TAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Heroin; adverse effects; Image Processing, Computer-Assisted; Prefrontal Cortex; drug effects; metabolism; Proteome; metabolism; Proteomics; Rats; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Journal of Southern Medical University 2007;27(4):427-431
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain two-dimensional gel electrophoresis maps of the prefrontal cortex (PFC) proteins of normal and heroin-addicted rats for identifying the differentially expressed proteins in the addicted rats.
METHODSRat models of heroin addiction were established, and the proteins in the PFC underwent two-dimensional gel electrophoresis with immobiline pH gradient isoelectric focusing as the first and vertical SDS-PAGE as the second dimension. ImageMaster 2D 5.0 analysis software, matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-TOF-MS as well as NCBInr database searching were performed to separate and identify the proteome in the rat PFC.
RESULTSSeven protein spots to represent 5 proteins were identified by PMF and MALDI-TOF-TOF-MS. Among those proteins, glucose-regulated protein (58 kD) and 26 S proteasome subunit p40.5 existed only in heroin-addicted rats, in which ATP synthase D chain was down-regulated. Ndufa10 and Eno1 were present in both groups, but their molecular mass and pI were different.
CONCLUSIONImmobilized pH gradient two-dimensional gel electrophoresis allows good reproducibility in separation and identification of the proteome in the PDF of heroin-addicted rats, which facilitates further investigation of pathogenic mechanisms of heroin addiction and central nerve injury.
