Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells cultured with interleukin-2.
- Author:
Hong CHANG
1
;
Wei LUO
;
Li MA
;
Ming-qian ZHOU
;
Qian WEN
;
Yuan-bin WU
;
Yu-xian HUANG
;
Kun-yuan GUO
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Complementarity Determining Regions; genetics; Genetic Drift; Humans; Interleukin-2; metabolism; Leukocytes, Mononuclear; metabolism; Receptors, Antigen, T-Cell, alpha-beta; genetics; T-Lymphocytes; immunology; metabolism
- From: Journal of Southern Medical University 2007;27(4):433-435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor beta (TCRbeta) chain variable region in T cells of healthy volunteers cultured with interleukin-2 (IL-2).
METHODST cells were isolated from the peripheral blood and cultured in vitro in the presence of IL-2. The non-specific killing effect of the cells was analyzed by LDH releasing assay, and the distribution of TCRbeta chain CDR3 in healthy volunteers by immunoscope spectratyping method to evaluate the clonality of the T cells.
RESULTSThe results showed Gaussian distribution of TCR Vbeta gene CDR3 in healthy volunteers. The T cell cultured with IL-2, however, displayed some anomalous and oligoclonal expansion in different TCR Vbeta families without killing effect against nasophargngal carcinoma cell line CNE2.
CONCLUSIONIL-2 may affect TCRbeta chain CDR3 distribution in T cells cultured in vitro.
