Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry for proteomic analysis of seminal plasma in patients with delayed semen liquefaction.
- Author:
Ling SUN
1
;
Jie BAI
;
Shi-ling CHEN
;
Zhuo-lin QIU
;
Wei-qing ZHANG
;
Jie YANG
;
Fu-qi XING
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Humans; Infertility, Male; metabolism; Male; Proteomics; methods; Semen; chemistry; Seminal Plasma Proteins; analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Journal of Southern Medical University 2007;27(4):442-444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the differentially expressed proteins in seminal plasma between subjects with normal fertility and those with delayed semen liquefaction by proteomic techniques.
METHODSSemen samples of 6 patients with delayed semen liquefaction and 11 subjects with normal fertility (control group) were collected. Seminal plasma were separated and examined with surface-enhanced laser desorption/ionization time of flight mass spectrometry to compare the protein expressions between the two groups.
RESULTSCompared with normal control group, 19 proteins were differentially expressed in the patients including 6 proteins with significant differences between the two groups, with m/z of 8696.621, 9770.076, 9512.309, 10202.64, 2941.903, and 9617.759, respectively (P<0.01).
CONCLUSIONScreening of differentially expressed proteins in seminal plasma may help understand the mechanism of delayed semen liquefaction for its potential clinical intervention.
