Specific small interfering RNAs inhibit replication of hepatitis B virus.
- Author:
Jing JIAO
1
,
2
;
Hong CAO
;
Mei-juan ZHOU
;
Zhi-hua LIU
;
Zhen-hua DING
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Hepatitis B Surface Antigens; analysis; Hepatitis B virus; drug effects; physiology; Humans; RNA, Messenger; analysis; RNA, Small Interfering; pharmacology; RNA, Viral; analysis; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Virus Replication; drug effects
- From: Journal of Southern Medical University 2007;27(4):465-467
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain specific small interfering RNAs (siRNA) for hepatitis B virus (HBV) and evaluate their interfering effect.
METHODSThree siRNAs were transfected into HepG2.2.15 cells, and the amount of HBV mRNA in the cell culture medium was quantified with real-time fluorescence quantitative RT-PCR. HBsAg in the culture media was assayed with Western blotting at different time points after transfection.
RESULTSThe cells transfected with specific siRNAs showed decreased levels of HBV mRNA and HBsAg (P<0.05), but those with nonspecific siRNA transfection as the negative control did not show such changes (P>0.05).
CONCLUSIONSpecific siRNA can significantly inhibit protein expression and mRNA synthesis of HBV in HepG2.2.15 cells in vitro.
