Toxic effects of CD-TK double suicidal gene system against prostate carcinoma cells.
- Author:
Wen-hui ZHU
1
;
Wan-long TAN
;
He HUANG
;
Xiang-hua SHI
;
Yi XIE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cytosine Deaminase; pharmacology; Genes, Transgenic, Suicide; Genetic Therapy; methods; Genetic Vectors; Humans; Male; Prostatic Neoplasms; therapy; Reverse Transcriptase Polymerase Chain Reaction; Thymidine Kinase; pharmacology; Transfection
- From: Journal of Southern Medical University 2007;27(4):479-481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the toxic effects of the CD-TK fusion gene systems against prostate carcinoma cell line RM-1 for assessing the value of suicidal gene therapy for prostate carcinoma.
METHODSCD-TK fusion gene and green fluorescent protein (GFP) gene were transfected into RM-1 cells through adenovirus vectors. RT-PCR was used to demonstrate successful transfection and transcription of the suicidal genes. The toxic effects of 5-FC and GCV used alone or in combination on the transfected cells were observed by MTT assay, with the non-transfected RM-1 cells serving as control.
RESULTSCytotoxic activity of CD/5-FC and TK/GCV systems against RM-1 cells was observed, and combined treatment with the two drugs resulted in significantly lowered survival of CD-TK-expressing cells (P<0.05). After exposure to 5-FC and GCV for 72 h, the survival rate of the transfected cells decreased to 71.56% and 47.27%, respectively, and their combined use resulted in a survival rate as low as 18.46%.
CONCLUSIONCD-TK fusion double suicidal gene system can produce significantly stronger toxic effect against RM-1 cells in vitro than either of suicidal genes.