High-performance liquid chromatography for determining plasma alpha-lipoic acid in New Zealand rabbits.
- Author:
Yue-lian YANG
1
;
Hong LIU
;
Liang SUN
;
Zhong-yi ZHANG
;
Ai-min JI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; methods; Rabbits; Thioctic Acid; blood
- From: Journal of Southern Medical University 2007;27(4):548-549
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determining the content of alpha-lipoic acid in New Zealand rabbit plasma.
METHODSAlpha-lipoic acid in the plasma samples was purified by solid-phase extractor and analyzed on an HYPERSIL C18 column with isocratic mobile phase consisting of potassium dihydrogen phosphate-acetonitrile (50:50, v/v) at a flow rate of 1.0 ml/min and detection wavelength of 230 nm.
RESULTSThe standard curve was linear in the range of 5-100 microg/L (r=1) and the average recovery was 77.4%-82.1%. The relative standard deviations of intra-day and inter-day assay were within 1.5%-8.9%.
CONCLUSIONThe method is sensitive, accurate and simple for determining plasma alpha-lipoic acid levels in New Zealand rabbits.
