Expression of alpha7-nAChR on rat hippocampal astrocytes in vivo and in vitro.
- Author:
Yan WANG
1
;
Zhong-yi ZHANG
;
Yong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Astrocytes; cytology; metabolism; Cells, Cultured; Glial Fibrillary Acidic Protein; analysis; Hippocampus; cytology; metabolism; Immunohistochemistry; Microscopy, Fluorescence; Rats; Rats, Sprague-Dawley; Receptors, Nicotinic; biosynthesis; alpha7 Nicotinic Acetylcholine Receptor
- From: Journal of Southern Medical University 2007;27(5):591-594
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of alpha7-nAChR on rat hippocampal astrocytes in vivo and in vitro.
METHODSRat hippocampus was isolated and cut into 30-microm cryosections with anti-GFAP antibody staining followed by staining for alpha7-nAChR. The cultured astrocytes obtained from newborn rat (1-2 days old) hippocampus were identified with GFAP, and the expression of alpha7-nAChR was measured by fluorescein isothcyanate-tagged alpha-bungarotoxin staining analysis and double immunolabeling.
RESULTSThe localization of alpha7-nAChR was visualized in green with fluorescein isothiocyanate labeled IgG, whereas that of GFAP in red with Texas red-labeled IgG in the hippocampal slices, and the yellow spots indicating colocalization of the two fluorescent probes was shown in a merging image. alpha-bungarotoxin-binding nicotinic receptors were clustered, and the colocalization of alpha7-nAChR and GFAP on the cultured hippocampal astrocytes was visualized with the two fluorescent probes.
CONCLUSIONThe expression of alpha7-nAChR is identified on hippocampal astrocytes in vivo and in vitro.
