Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine.
- Author:
Chao LIU
1
;
Ling CHEN
;
Xiao-hui CHEN
;
Chang-hui LIU
;
Hui-jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromosomes, Human, Y; genetics; Forensic Medicine; methods; Gene Frequency; Genetic Variation; Genotype; Haplotypes; Humans; Male; Microsatellite Repeats; genetics; Polymorphism, Genetic; Population Groups
- From: Journal of Southern Medical University 2007;27(5):599-601
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the allele frequency distribution, gene diversity and haplotype diversity of 12 Y-specific short tandem repeats (STR) in Guangzhou Han population and evaluate their forensic application.
METHODSTwelve Y-STR loci in 401 unrelated male Han individuals in Guangzhou were amplified with PowerPlex(R) Y System, and the PCR products were detected with 3100 Genetic Analyzer.
RESULTSThe data of allele distribution and gene diversity of the 12 Y-STR loci were obtained from these individuals. A total of 398 haplotypes were observed and the overall haplotype diversity for the 12 Y-STR loci was 0.99997. The DNA samples of 13 different species of animals were amplified and no specific products were observed. Different tissues from the same individual exhibited the same Y-STR haplotype.
CONCLUSIONThe 12 Y-STR loci exhibit high genetic polymorphism and are of important value in understanding of the human origin, individual identification for mixed male/female DNA samples and paternity identification.
