Hepatocyte growth factor enhances protein synthesis in cardiomyocytes exposed to gamma-ray irradiation.
- Author:
Shun-ying HU
1
;
Chao-ping FU
;
Hai-feng DUAN
;
Jin-long CHEN
;
Rong-liang WANG
;
Bin WU
;
Zi-kuan GUO
;
Guo-wei CHEN
;
Li-sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Cycle; drug effects; radiation effects; Cells, Cultured; Flow Cytometry; Gamma Rays; Green Fluorescent Proteins; genetics; metabolism; Hepatocyte Growth Factor; pharmacology; Microscopy, Fluorescence; Myocytes, Cardiac; cytology; metabolism; Protein Biosynthesis; drug effects; radiation effects; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2007;27(5):602-604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of hepatocyte growth factor (HGF) on protein synthesis in rat cardiomyocytes exposed to gamma-ray irradiation.
METHODSPrimary cultured cardiomyocytes were irradiated with single-dose (20 Gy) gamma ray in the absence or presence of HGF (40 ng/ml) added in the cell culture 3 h before the exposure. Forty-eight hours after irradiation, the total cellular protein was measured and cell cycle analyzed by flow cytometry. The cardiomyoctes were also infected with AdGFP 48 h after irradiation and the fluorescence intensity of the green fluorescence protein (GFP) in the cells determined by flow cytometry 48 h after infection.
RESULTSThe protein synthesis was decreased significantly in the irradiated cardiomyocytes as compared with the control group (P<0.01), but was remedied significantly by incubation of the cells with HGF before the exposure (P<0.05). Flow cytometry revealed much lower mean fluorescence intensity (MFI) of GFP in irradiated cardiomycytes than in cells without the exposure (P<0.01); The MFI was higher in HGF-treated cardiomyocytes than in cells without HGF treatment following the exposure (P<0.01).
CONCLUSIONGamma ray irradiation inhibits protein synthesis in cardiomyocytes, and HGF may attenuate this effect of gamma ray exposure for cardiomyocyte protection.
