Apoptosis induction of rhesus peripheral blood lymphocytes.
- Author:
Li-pei FAN
1
;
Er-wei SUN
;
Xiao-peng YUAN
;
Xiao LU
;
Hai-qin WANG
;
Zhan-guo LIU
;
Min LI
;
Ming ZHAO
;
Shi-zhen ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; radiation effects; Cells, Cultured; Dose-Response Relationship, Radiation; Flow Cytometry; Gamma Rays; Leukocytes, Mononuclear; cytology; radiation effects; Lymphocytes; cytology; radiation effects; Macaca mulatta; Male; Time Factors; Ultraviolet Rays; X-Rays
- From: Journal of Southern Medical University 2007;27(5):628-630
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for inducing apoptosis of rhesus peripheral blood lymphocytes (PBLs).
METHODSRhesus PBLs were irradiated with X-ray, (60)Co gamma-rays and ultraviolet (UVC254 nm), respectively, and the cell apoptosis was evaluated with flow cytometry using annexin-V staining and propidium iodide staining.
RESULTSX-ray and (60)Co gamma-ray irradiation induced only low apoptotic rates of the PBLs, and UVC resulted in the highest apoptotic rate of about 60%. UVC irradiation of the PBLs in RPMI supplemented with 10% heat-inactivated fetal calf serum for 60 min at a distance of 20 cm led to an early apoptotic rate of 58.85% and necrotic rate of 11.5%. The apoptotic rate of PBLs increased in a dose- and time-dependent fashion.
CONCLUSIONFor inducing apoptosis of the rhesus PBLs, UVC can be more effective than X-ray and (60)Co gamma-ray. The highest apoptotic rate can be achieved when the rhesus PBLs in RPMI supplemented with 10% heat-inactivated fetal calf serum are exposed to UVC for 60 min at the distance of 20 cm.
